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  • 标题:Nuclear expansion and pore opening are instant signs of neuronal hypoxia and can identify poorly fixed brains
  • 本地全文:下载
  • 作者:Anisa Dehghani ; Hulya Karatas ; Alp Can
  • 期刊名称:Scientific Reports
  • 电子版ISSN:2045-2322
  • 出版年度:2018
  • 卷号:8
  • 期号:1
  • 页码:14770
  • DOI:10.1038/s41598-018-32878-1
  • 语种:English
  • 出版社:Springer Nature
  • 摘要:The initial phase of neuronal death is not well characterized. Here, we show that expansion of the nuclear membrane without losing its integrity along with peripheralization of chromatin are immediate signs of neuronal injury. Importantly, these changes can be identified with commonly used nuclear stains and used as markers of poor perfusion-fixation. Although frozen sections are widely used, no markers are available to ensure that the observed changes were not confounded by perfusion-induced hypoxia/ischemia. Moreover, HMGB1 was immediately released and p53 translocated to mitochondria in hypoxic/ischemic neurons, whereas nuclear pore complex inhibitors prevented the nuclear changes, identifying novel neuroprotection targets.
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