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  • 标题:LED based real-time survival bioassays for nematode research
  • 本地全文:下载
  • 作者:Satish Kumar Rajasekharan ; Chaitany Jayaprakash Raorane ; Jintae Lee
  • 期刊名称:Scientific Reports
  • 电子版ISSN:2045-2322
  • 出版年度:2018
  • 卷号:8
  • 期号:1
  • 页码:11531
  • DOI:10.1038/s41598-018-30016-5
  • 语种:English
  • 出版社:Springer Nature
  • 摘要:) of a toxic drug, whereas they responded rapidly to blue (450-490 nm) and ultraviolet (UV) (100-400 nm) LED lights. Furthermore, paralyzed nematodes responded in less than 5 seconds to a LED pulse. The response to the LED stimulus was distinctively noted in C. elegans dauers, which squirmed away from illuminated sites within seconds. LED produced an incoherent beam, and uniformly distributed light across the sampling area. In conclusion, this method is more accurate than the conventional counting techniques, and enables us to differentiate paralyzed and dead nematodes virtually in real-time. Furthermore, this technique would appear to be suitable for incorporating a motion-sensor based automated system.
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