摘要：Agricultural production combined with planting and breeding, which can reduce chemical fertilizer and pesticide applications, reduce losses due to natural disasters, and improve the output and quality of agricultural products, is an important way to achieve green, circular and efficient production. To assess effects on soil bacterial community structure, a vegetable-eel-earthworm integrated planting and breeding platform (VEE-IPBP) combined with experiment planting was established at Chongming Island, Shanghai and compared to traditional planting. High-throughput sequencing to reveal soil bacterial community structure was performed on samples collected at 0, 3 and 6 years after implementation of the two models. Over time, the Shannon index first increased and then decreased in the VEE-IPBP system and was reduced by 3.2% compared to the traditional planting (In the same time and space scale, the single-degree planting method of dryland vegetables under mechanical cultivation is adopted) (p < 0.05). In contrast, Chao and Ace indices were increased by 2.4% and 3.2%. Thus, soil bacterial diversity was markedly different in the two planting models. The abundance of Proteus, Cyanophyta and Cyanophyta in soil increased after 6 years, and the proportion of Lysinibacillus increased significantly, contributing to improvement in soil disease resistance. Redundancy analysis (RDA) showed that the soil pH and water content were the main factors influencing the change in soil bacterial community structure in the two planting models, and the dominant species of soil bacteria were Lysobacter and Bacillus.
