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  • 标题:An Oil-Free Picodrop Bioassay Platform for Synthetic Biology
  • 本地全文:下载
  • 作者:Christian A. Siltanen ; Russell H. Cole ; Sean Poust
  • 期刊名称:Scientific Reports
  • 电子版ISSN:2045-2322
  • 出版年度:2018
  • 卷号:8
  • 期号:1
  • 页码:7913
  • DOI:10.1038/s41598-018-25577-4
  • 语种:English
  • 出版社:Springer Nature
  • 摘要:Droplet microfluidics enables massively-parallel analysis of single cells, biomolecules, and chemicals, making it valuable for high-throughput screens. However, many hydrophobic analytes are soluble in carrier oils, preventing their quantitative analysis with the method. We apply Printed Droplet Microfluidics to construct defined reactions with chemicals and cells incubated under air on an open array. The method interfaces with most bioanalytical tools and retains hydrophobic compounds in compartmentalized reactors, allowing their quantitation.
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