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  • 标题:Tunable Ampere phase plate for low dose imaging of biomolecular complexes
  • 本地全文:下载
  • 作者:Amir H. Tavabi ; Marco Beleggia ; Vadim Migunov
  • 期刊名称:Scientific Reports
  • 电子版ISSN:2045-2322
  • 出版年度:2018
  • 卷号:8
  • 期号:1
  • 页码:5592
  • DOI:10.1038/s41598-018-23100-3
  • 语种:English
  • 出版社:Springer Nature
  • 摘要:A novel device that can be used as a tunable support-free phase plate for transmission electron microscopy of weakly scattering specimens is described. The device relies on the generation of a controlled phase shift by the magnetic field of a segment of current-carrying wire that is oriented parallel or antiparallel to the electron beam. The validity of the concept is established using both experimental electron holographic measurements and a theoretical model based on Ampere's law. Computer simulations are used to illustrate the resulting contrast enhancement for studies of biological cells and macromolecules.
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