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  • 标题:Thermal stabilization of the deglycating enzyme Amadoriase I by rational design
  • 本地全文:下载
  • 作者:Federica Rigoldi ; Stefano Donini ; Francesca Giacomina
  • 期刊名称:Scientific Reports
  • 电子版ISSN:2045-2322
  • 出版年度:2018
  • 卷号:8
  • 期号:1
  • 页码:3042
  • DOI:10.1038/s41598-018-19991-x
  • 语种:English
  • 出版社:Springer Nature
  • 摘要:(55.3 °C and 60.6 °C, respectively) compared to the wild-type enzyme (52.4 °C). Moreover, SS17 shows clear hyperstabilization, with residual activity up to 95 °C, whereas the wild-type enzyme is fully inactive at 55 °C. Our computational screening method can therefore be considered as a promising approach to expedite the design of thermostable enzymes.
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