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  • 标题:Rapid one-step biotinylation of biological and non-biological surfaces
  • 本地全文:下载
  • 作者:Stephen Henry ; Eleanor Williams ; Katie Barr
  • 期刊名称:Scientific Reports
  • 电子版ISSN:2045-2322
  • 出版年度:2018
  • 卷号:8
  • 期号:1
  • 页码:2845
  • DOI:10.1038/s41598-018-21186-3
  • 语种:English
  • 出版社:Springer Nature
  • 摘要:We describe a rapid one-step method to biotinylate virtually any biological or non-biological surface. Contacting a solution of biotin-spacer-lipid constructs with a surface will form a coating within seconds on non-biological surfaces or within minutes on most biological membranes including membrane viruses. The resultant biotinylated surface can then be used to interact with avidinylated conjugates, beads, vesicles, surfaces or cells.
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