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  • 标题:Dynamic cellular maps of molecular species: Application to drug-target interactions
  • 本地全文:下载
  • 作者:Carolina García ; Alejandro Losada ; Miguel A. Sacristán
  • 期刊名称:Scientific Reports
  • 电子版ISSN:2045-2322
  • 出版年度:2018
  • 卷号:8
  • 期号:1
  • 页码:1140
  • DOI:10.1038/s41598-018-19694-3
  • 语种:English
  • 出版社:Springer Nature
  • 摘要:) as fluorescence tracer. Using the proposed methodology, we were able to follow in real time the formation and relative distribution of two sets of APL-target complexes in live cells, revealing two distinct patterns of behavior for HeLa-wt and APL resistant HeLa-APL-R cells. The information obtained may complement and facilitate the design of new experiments and the global interpretation of the results obtained with other biochemical and cell biology methods, as well as possibly opening new avenues of study to decipher the mechanism of action of new drugs.
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