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  • 标题:Evaluation of two enzyme-linked immunosorbent assays for the detection of antibodies against equine arteritis virus
  • 本地全文:下载
  • 作者:Hiroshi BANNAI ; Manabu NEMOTO ; Koji TSUJIMURA
  • 期刊名称:Journal of Equine Science
  • 印刷版ISSN:1340-3516
  • 电子版ISSN:1347-7501
  • 出版年度:2018
  • 卷号:29
  • 期号:4
  • 页码:111-115
  • DOI:10.1294/jes.29.111
  • 语种:English
  • 出版社:Japanese Society of Equine Science
  • 摘要:

    In order to establish an efficient system for serological diagnosis of equine viral arteritis in Japan, we compared enzyme-linked immunosorbent assays (ELISAs) provided by two manufacturers (Nisseiken Co., Ltd., Tokyo, Japan, and VMRD Inc., Pullman, WA, U.S.A.) by testing a series of horse sera. The results revealed that 159 of 160 virus-neutralizing (VN) antibody-positive serum samples were positive in both the Nisseiken-ELISA and VMRD-ELISA. Of the VN-negative sera (n=157), 134 and 154 samples were negative in the Nisseiken-ELISA and VMRD-ELISA, respectively. Sensitivity was 99.4% for both the Nisseiken-ELISA and VMRD-ELISA. The specificity of the VMRD-ELISA (98.1%) was significantly higher than that of the Nisseiken-ELISA (85.4%, P<0.05). The diagnostic performance of the VMRD-ELISA was superior to that of the Nisseiken-ELISA because of this greater specificity.

  • 关键词:ELISA;EVA;sensitivity;specificity
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