期刊名称：Potravinarstvo : Scientific Journal for Food Industry
印刷版ISSN：1338-0230
电子版ISSN：1337-0960
出版年度：2016
卷号：10
期号：1
页码：494-498
DOI：10.5219/646
语种：English
出版社：Association HACCP Consulting
摘要：Methods of in vitro conservation offer a number of advantages for endangered species preservation. Crambe tataria Sebeók biochemistry study (fatty acid (FA) composition, antioxidant activity (AOA), polyfructan and total soluble protein content) is fairly importaint and could show the potential value of this species in agriculture, Food and Chemical Industry or pharmacology including its use as a source of valuble genetic material and could lead to new promising sources of biofuel discovery. Also, comparison of in vitro and in vivo cultured plants could point out to the effect of in vitro culture methods on plants biochemical composition Fatty acid (FA) content was determined using Gas chromatography-mass spectrophotometry (GC/MS) of fatty acid ethers. Antioxidant activity was determined using DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging method. Total soluble protein content was measured using Bradford method and polyfructan content determination was based upon ketosugars ability to color in the acidic environment with resorcinol. Plants that were grown under in vitro and in vivo conditions and seeds were used in this research. Obtained data showed that C. tataria plants had high AOA and total soluble protein content along with high total FA content along with high content of α-Linolenic acid and absence of erucic acid. Difference in biochemical composition between plants grown in aseptic and not aseptic conditions was shown.
其他摘要：Methods of in vitro conservation offer a number of advantages for endangered species preservation. Crambe tataria Sebeók biochemistry study (fatty acid (FA) composition, antioxidant activity (AOA), polyfructan and total soluble protein content) is fairly importaint and could show the potential value of this species in agriculture, Food and Chemical Industry or pharmacology including its use as a source of valuble genetic material and could lead to new promising sources of biofuel discovery. Also, comparison of in vitro and in vivo cultured plants could point out to the effect of in vitro culture methods on plants biochemical composition Fatty acid (FA) content was determined using Gas chromatography-mass spectrophotometry (GC/MS) of fatty acid ethers. Antioxidant activity was determined using DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging method. Total soluble protein content was measured using Bradford method and polyfructan content determination was based upon ketosugars ability to color in the acidic environment with resorcinol. Plants that were grown under in vitro and in vivo conditions and seeds were used in this research. Obtained data showed that C. tataria plants had high AOA and total soluble protein content along with high total FA content along with high content of α-Linolenic acid and absence of erucic acid. Difference in biochemical composition between plants grown in aseptic and not aseptic conditions was shown.
关键词：threatened species; in vitro culture; GC/MS mass spectrophotometry; fatty acids
其他关键词：threatened species;in vitro culture;GC/MS mass spectrophotometry;fatty acids
