摘要：This investigation aims to evaluate the influence of high hydrostatic pressure (HHP) treatment after germination on soyasaponin conversion and physiological activity in black soybean [Glycine max (L.)]. Black soybean was germinated for 4 days and subjected to 24 h at 0.1-150 MPa; the crude soyasaponin extracts were then evaluated for anti-inflammatory and anti-obesity activity. The highest crude soyasaponin content of 57.85 mg/g was found in germinated black soybean treated at 100 MPa. Soyasaponin Bb and Bc were the major B-group soyasaponins in raw black soybean; their contents in raw and germinated black soybean increased after HHP treatment. The lipid accumulation rate during 3T3-L1 adipocyte differentiation in germinated HHP-treated black soybean ranged from 93.25-65.95% at the concentration of 400 μg/mL. The highest anti-obesity effect was observed after germination and 150-MPa treatment. Black soybean sprout extracts treated at 150 MPa significantly inhibited the lipopolysaccharide-induced expression of inflammatory markers by RAW 264.7 macrophages.
