摘要：Background and Objective:Land-filling is considered as a common method for disposal of Municipal Solid Waste (MSW) and the landfills are regarded as repository of various groups of important microbes growing on organic waste materials. In temperate regions due to lower environmental temperatures the landfill sites are inhabited by a distinct group of bacteria that utilize the wastes as energy sources through the secretion of important cold active enzymes. Hence, the present study was taken to examine physicochemical and bacteriological characterization of organic municipal waste samples collected form landfill site. Materials and Methods:A total of 15 waste samples mixed with soil were randomly collected from the landfill site. The waste samples were analyzed for various physicochemical parameters by following available standard methodology. The isolation of bacteria was done by following serial dilution and spread plate method at temperature of 15°C, pH 7.0 and incubation period of 48 h. Results:The results of the study revealed that the landfill wastes at the surface were partially decomposed by the presence of diverse group of cold active bacterial isolates. The morphological characterization of the isolates revealed that most of bacteria belonged to genus Bacillus (gram+) majority of which produced two important types of cold active enzymes (CMCases, proteases) in response to different substrates (carboxymethyl cellulose and skim milk) under in vitro conditions. Conclusion:It was concluded that the landfill site acted as the repository of unique group of cold active bacteria most of which showed potential of producing multiple enzymes. The study presents a platform for further scientific investigation through microbial profiling of landfill site that may lead to discovery of some novel microbes of high enzyme producing potential of scientific importance.
关键词：MSW; landill; cold active bacteria; decomposition; enzyme activity; Bacillus sp.
