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  • 标题:RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) PROFILES OF SEVERAL ISOLATES OF Ganoderma spp. FROM BANYUMAS, CENTRAL JAVA, INDONESIA
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  • 作者:Nuniek Ina Ratnaningtyas ; Agus Hery Susanto ; Ana Yulia
  • 期刊名称:BIOTROPIA
  • 印刷版ISSN:1907-770X
  • 出版年度:2019
  • 卷号:26
  • 期号:2
  • 页码:84-103
  • DOI:10.11598/btb.2019.26.2.884
  • 出版社:SEAMEO BIOTROP
  • 摘要:A study on the RAPD profiles of several isolates of Ganoderma spp. from Banyumas Regency, Central Java, Indonesia and their genetic inter-relationship based on RAPD markers was conducted using a survey method, where random sampling technique was applied. Collected Ganoderma spp. fruiting bodies were first morphologically characterized, after which RAPD analysis utilizing four random primers, i.e. OPC-1, OPC-2, OPC-4 and OPC-5 was carried out. The results revealed that the four primers used generated polymorphic bands toward 10 samples with polymorphism level of 100%, showing absolutely high genetic diversity. The level of genetic similarity ranged between 0.48 and 0.82, indicating moderate similarities among samples. A constructed dendogram indicated that the isolates of Ganoderma spp. were grouped into three clusters at a coefficient of 0.63, but neither according to geographical locations nor growth substrates.
  • 关键词:Banyumas; Ganoderma spp;isolates; RAPD .
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