摘要：Purpose: The imbalance between the formation of
reactive oxygen species and antioxidant capacity of
the body is known as oxidative stress. Exposition of
the skin to free radicals, the origin of the internal and
external causes activation of multiple mechanisms to
eliminate them and prevent in this way the
development of oxidative stress. The aim of this
experiment was examining what changes are taking
place in the antioxidant barrier of unwounded
healthy skin of rats, who are on a high amino acids
diet for 7 and 14 days at administered doses of 0.3
and 0.5 g/kg body weight.
Materials and Methods: The study was performed
on male Wistar rats divided into 5 groups: 1. control
(standard feed), 2. high amino acid diet (WPC-80
80% whey protein) administered for 7 days at a dose
of 0.3g/kg of body weight, 3. WPC-80 for 7 days at
a dose of 0.5g/kg of body weight, 4. WPC-80 for 14
days at a dose of 0.3g/kg of body weight, 5. WPC80
for 14 days at a dose of 0.5g/kg of body weight.
The concentration of superoxide dismutase 2 and 3,
the concentration of catalase specific activity of
glutathione peroxidase, the concentration of
glutathione and total protein content were
determined.
Results: The supplementation of the standard diet by
the preparation of WPC-80 administered in a dose
0.5 g/kg body weight for 14 days containing
methionine and cysteine (essential amino acids
involved in the formation of glutathione),
significantly increases the concentration of reduced
glutathione.
Conclusions: Enrichment of a standard diet with
WPC-80 caused by the significant increases of nonenzymatic
antioxidant.
