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  • 标题:Molecular characterization of Hymenolepis nana based on nuclear rDNA ITS2 gene marker
  • 本地全文:下载
  • 作者:Mojtaba Shahnazi ; Majid Zarezadeh Mehrizi ; Safar Ali Alizadeh
  • 期刊名称:African Health Sciences
  • 印刷版ISSN:1680-6905
  • 电子版ISSN:1729-0503
  • 出版年度:2019
  • 卷号:19
  • 期号:1
  • 页码:1346-1352
  • DOI:10.4314/ahs.v19i1.6
  • 出版社:Makerere University Medical School(Uganda)
  • 摘要:

    Introduction: Hymenolepis nana is a zoonotic tapeworm with widespread distribution. The goal of the present study was to identify the parasite in the specimens collected from NorthWestern regions of Iran using PCR-sequencing method.

    Methods: A total of 1521 stool samples were collected from the study individuals. Initially, the identification of hymenolepis nana was confirmed by parasitological method including direct wet-mount and formalin-ethyl acetate concentration methods. Afterward, PCR-sequencing analysis of ribosomal ITS2 fragment was targeted to investigate the molecular identification of the parasite.

    Results: Overall, 0.65% (10/1521) of the isolates were contaminated with H. nana in formalin-ethyl acetate concentration. All ten isolates were succefully amplified by PCR and further sequenced. The determined sequences were deposited in GenBank under the accession numbers MH337810 -MH337819.

    Conclusion: Our results clarified the presence of H. nana among the patients in the study areas. In addition, the molecular tech- nique could be accessible when the human eggs are the only sources available to identify and diagnose the parasite.

  • 关键词:Hymenolepis nana; rDNAITS2; PCR; Iran.
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