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  • 标题:Triiodothyronine (T3) upregulates the expression of proto-oncogene TGFA independent of MAPK/ERK pathway activation in the human breast adenocarcinoma cell line, MCF7
  • 其他标题:Triiodothyronine (T3) upregulates the expression of proto-oncogene TGFA independent of MAPK/ERK pathway activation in the human breast adenocarcinoma cell line, MCF7
  • 本地全文:下载
  • 作者:Silva, Tabata M. ; Moretto, Fernanda C. F. ; Sibio, Maria T. De
  • 期刊名称:Archives of Endocrinology and Metabolism
  • 印刷版ISSN:2359-3997
  • 出版年度:2019
  • 卷号:63
  • 期号:2
  • 页码:142-147
  • DOI:10.20945/2359-3997000000114
  • 出版社:Archives of Endocrinology and Metabolism
  • 摘要:ABSTRACT Objective: To verify the physiological action of triiodothyronine T3 on the expression of transforming growth factor α (TGFA) mRNA in MCF7 cells by inhibition of RNA Polymerase II and the MAPK/ERK pathway Materials and methods: The cell line was treated with T3 at a physiological dose (10−9M) for 10 minutes, 1 and 4 hour (h) in the presence or absence of the inhibitors, α-amanitin (RNA polymerase II inhibitor) and PD98059 (MAPK/ERK pathway inhibitor). TGFA mRNA expression was analyzed by RT-PCR. For data analysis, we used ANOVA, complemented with the Tukey test and Student t-test, with a minimum significance of 5%. Results: T3 increases the expression of TGFA mRNA in MCF7 cells in 4 h of treatment. Inhibition of RNA polymerase II modulates the effect of T3 treatment on the expression of TGFA in MCF7 cells. Activation of the MAPK/ERK pathway is not required for T3 to affect the expression of TGFA mRNA. Conclusion: Treatment with a physiological concentration of T3 after RNA polymerase II inhibition altered the expression of TGFA. Inhibition of the MAPK/ERK pathway after T3 treatment does not interfere with the TGFA gene expression in a breast adenocarcinoma cell line.
  • 关键词:Thyroid hormone; nongenomic actions; gene expression; breast cancer.
  • 其他关键词:Thyroid hormone;nongenomic actions;gene expression;breast cancer
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