The study was conducted to determine the effect of the fungi Trichoderma harzianum and Trichoderma koningii loaded on alginic acid and 2% CMC in sterile and non-sterilized soil, treated with mineral fertilizer and organic fertilizer against pathogenic fungus Fusarium oxysporum on the plant of tomato in the field. The best results of the fungus T.harzianum and T.koningii loaded with alginic acid in sterile soil with organic compost against pathogenic fungus and improved plant growth. Where the growth indicators for the length of the plant were 186.66, 186.33, fruits weight were 3231.50, 3592.00 and number of fruits were 25.00, 21.00 for the two fungi T.harzianum and T.koningii respectively. While chlorophyll 69.33 and 69.66, nitrogen 6.57 and 6.54 and phosphor 0.591 and 0.585 for T.harzianum and T.koningii respectively, also the fungus T.harzianum and T.koningii loaded with alginic acid in sterile soil with organic compost improve photosynthetic process the results of photosynthesis 16.75 and 16.29 and respiration 6.53 and 7.25 respectively. This study was designed to determine the effect of bio fungus T.harzianum and T.koningii and their filters against the pathogen F.oxysporum in the laboratory and investigate the effect of T.harzianum and T. koningii loaded on alginic acid and 2% CMC on growth indicators.