The present study was conducted to characterize various bioactive phytoconstituents from blueberry, chokeberry and strawberry leaves using FTIR spectroscopy. Attenuated total reflectance (ATR) spectra of plant leaves display complex absorption features related to organic constituents of leaf surfaces. The spectra can be recorded rapidly without special sample preparation. This paper explores sources of ATR spectral variation in leaves, including compositional, positional variations.