期刊名称：Journal of Advanced Veterinary and Animal Research
印刷版ISSN：2311-7710
出版年度：2019
卷号：6
期号：2
页码：236-240
DOI：10.5455/javar.2019.f338
出版社：Bangladesh Agricultural University, Faculty of Veterinary Science
摘要：Objective: This study aimed to explore the phytochemical constituents and anthelmintic activities of four Cassia spp. leaves against Haemonchus contortus . Materials and Methods: The extracts were prepared from four species of Cassia spp. ( C. siamea , C. fistula , C. surattensis , and C. spectabilis ). Phytochemical screening of the extract was done based on the Harborne method. Evaluation of the anthelmintic activities against H . contortus was done in vitro using infective larvae (L3) migration inhibition assay (LMIA). Measurement of larvae migrating was conducted through a nylon filter with a pore size of 20 μm. The doses of Cassia spp. extract implemented were 25, 50, 100, and 200 mg/ml. Results: Tannins, alkaloids, phenol hydroquinone, flavonoids, steroids, triterpenoids, and saponins were present in all the extracts, whereas alkaloids were absent in C . fistula . No triterpenoids were found in C. surattensis and C. spectabilis . Movement of H . contortus larvae was significantly inhibited after exposure to Cassia extracts at various dosage levels ( p < 0.05). The test results using LMIA on L3 H . contortus showed the lowest inhibition in the negative control. Among the species of Cassia , the C . surattensis (at 200 mg/ml) showed the highest ( p < 0.05) inhibition level on the larvae. The latter result corresponded to the effect of albendazole. Conclusion: Compared to other Cassia spp., C . surattensis exhibited the highest inhibition against L3 H . contortus . However, the inhibition effect of C . surattensis was still lower as compared to albendazole. J. Adv. Vet. Anim. Res., 6(2): 236-240, June 2019
关键词：Anthelmintic effect; C. siamea; C. fistula; C. surattensis; C. spectabilis; H. contortus
