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  • 标题:A quantitative ratiometric sensor for time-resolved analysis of auxin dynamics
  • 本地全文:下载
  • 作者:Sabrina Wend ; Cristina Dal Bosco ; Michael M. Kämpf
  • 期刊名称:Scientific Reports
  • 电子版ISSN:2045-2322
  • 出版年度:2013
  • 卷号:3
  • 期号:1
  • DOI:10.1038/srep02052
  • 语种:English
  • 出版社:Springer Nature
  • 摘要:Time-resolved quantitative analysis of auxin-mediated processes in plant cells is as of yet limited. By applying a synergistic mammalian and plant synthetic biology approach, we have developed a novel ratiometric luminescent biosensor with wide applicability in the study of auxin metabolism, transport, and signalling. The sensitivity and kinetic properties of our genetically encoded biosensor open new perspectives for the analysis of highly complex auxin dynamics in plant growth and development.
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