首页    期刊浏览 2024年11月11日 星期一
登录注册

文章基本信息

  • 标题:A rapid and robust tri-color flow cytometry assay for monitoring malaria parasite development
  • 本地全文:下载
  • 作者:Benoît Malleret ; Carla Claser ; Alice Soh Meoy Ong
  • 期刊名称:Scientific Reports
  • 电子版ISSN:2045-2322
  • 出版年度:2011
  • 卷号:1
  • 期号:1
  • DOI:10.1038/srep00118
  • 语种:English
  • 出版社:Springer Nature
  • 摘要:

    Microscopic examination of Giemsa-stained thin blood smears remains the gold standard method used to quantify and stage malaria parasites. However, this technique is tedious, and requires trained microscopists. We have developed a fast and simple flow cytometry method to quantify and stage, various malaria parasites in red blood cells in whole blood or in vitro cultured Plasmodium falciparum. The parasites were stained with dihydroethidium and Hoechst 33342 or SYBR Green I and leukocytes were identified with an antibody against CD45. Depending on the DNA stains used, samples were analyzed using different models of flow cytometers. This protocol, which does not require any washing steps, allows infected red blood cells to be distinguished from leukocytes, as well as allowing non-infected reticulocytes and normocytes to be identified. It also allows assessing the proportion of parasites at different developmental stages. Lastly, we demonstrate how this technique can be applied to antimalarial drug testing.

    .

    © 2011 Macmillan Publishers Limited. All rights reserved

国家哲学社会科学文献中心版权所有