文章基本信息
- 标题：Tandem assembly of the epothilone biosynthetic gene cluster by in vitro site-specific recombination
- 本地全文：下载
- 作者：Lin Zhang ; Guoping Zhao ; Xiaoming Ding 等
- 期刊名称：Scientific Reports
- 电子版ISSN：2045-2322
- 出版年度：2011
- 卷号：1
- 期号：1
- DOI：10.1038/srep00141
- 语种：English
- 出版社：Springer Nature
- 摘要：
We describe a site-specific recombination-based tandem assembly (SSRTA) method for reconstruction of biological parts in synthetic biology. The system was catalyzed by Streptomyces phage φBT1 integrase, which belongs to the large serine recombinase subfamily. This one-step approach was efficient and accurate, and able to join multiple DNA molecules in vitro in a defined order. Thus, it could have applications in constructing metabolic pathways and genetic networks.
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