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  • 标题:Tracking of Normal and Malignant Progenitor Cell Cycle Transit in a Defined Niche
  • 本地全文:下载
  • 作者:Gabriel Pineda ; Kathleen M. Lennon ; Nathaniel P. Delos Santos
  • 期刊名称:Scientific Reports
  • 电子版ISSN:2045-2322
  • 出版年度:2016
  • 卷号:6
  • 期号:1
  • DOI:10.1038/srep23885
  • 语种:English
  • 出版社:Springer Nature
  • 摘要:While implicated in therapeutic resistance, malignant progenitor cell cycle kinetics have been difficult to quantify in real-time. We developed an efficient lentiviral bicistronic fluorescent, ubiquitination-based cell cycle indicator reporter (Fucci2BL) to image live single progenitors on a defined niche coupled with cell cycle gene expression analysis. We have identified key differences in cell cycle regulatory gene expression and transit times between normal and chronic myeloid leukemia progenitors that may inform cancer stem cell eradication strategies.
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