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  • 标题:“Calibration-on-the-spot”: How to calibrate an EMCCD camera from its images
  • 本地全文:下载
  • 作者:Kim I. Mortensen ; Henrik Flyvbjerg
  • 期刊名称:Scientific Reports
  • 电子版ISSN:2045-2322
  • 出版年度:2016
  • 卷号:6
  • 期号:1
  • DOI:10.1038/srep28680
  • 语种:English
  • 出版社:Springer Nature
  • 摘要:In order to count photons with a camera, the camera must be calibrated. Photon counting is necessary, e.g., to determine the precision of localization-based super-resolution microscopy. Here we present a protocol that calibrates an EMCCD camera from information contained in isolated, diffraction-limited spots in any image taken by the camera, thus making dedicated calibration procedures redundant by enabling calibration post festum, from images filed without calibration information.
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