摘要：In this study, the effects of La(3+) on the reactive oxygen species (ROS) and antioxidant metabolism were studied in the roots of rice (Oryza sativa L. cv Shengdao 16) exposed to increasing concentrations of La(3+) (0.05, 0.1, 0.5, 1.0, and 1.5 mM). The level of hydrogen peroxide, superoxide anion, and malondialdehyde was increased by 0.5, 1.0 and 1.5 mM La(3+), and the activity of catalase and peroxidase was increased by 0.05 and 0.1 mM La(3+). However, La(3+) treatments stimulated superoxide dismutase activity in the roots of rice seedlings at all tested concentrations. In addition, the probe 2',7'-dichlorofluorescein diacetate (H2DCF-DA) was used to investigate the instantaneous change of ROS in the root cells with the laser-scanning confocal microscopy. The result indicated that ROS level was declined after treated with 0.05 mM La(3+). The results showed that the appropriate concentration of La(3+) decreased the level of ROS, and hormetic effects on the antioxidant metabolism were found in the roots of rice exposed to 0.05, 0.1, 0.5, 1.0, and 1.5 mM La(3+).
