摘要：Global CO2 level presently recorded at 400 ppm is expected to reach 550 ppm in 2050, an increment likely to impact plant growth and productivity. Using targeted LC-MS and GC-MS platforms we quantified 229 and 29 metabolites, respectively in a time-course study to reveal short-term responses to different concentrations (1, 3, and 10 mM) of bicarbonate (HCO3(-)) under light and dark conditions. Results indicate that HCO3(-) treatment responsive metabolomic changes depend on the HCO3(-) concentration, time of treatment, and light/dark. Interestingly, 3 mM HCO3(-) concentration treatment induced more significantly changed metabolites than either lower or higher concentrations used. Flavonoid biosynthesis and glutathione metabolism were common to both light and dark-mediated responses in addition to showing concentration-dependent changes. Our metabolomics results provide insights into short-term plant cellular responses to elevated HCO3(-) concentrations as a result of ambient increases in CO2 under light and dark.
