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  • 标题:High-speed microscopy of continuously moving cell culture vessels
  • 本地全文:下载
  • 作者:Friedrich Walter Schenk ; Nicolai Brill ; Ulrich Marx
  • 期刊名称:Scientific Reports
  • 电子版ISSN:2045-2322
  • 出版年度:2016
  • 卷号:6
  • 期号:1
  • DOI:10.1038/srep34038
  • 语种:English
  • 出版社:Springer Nature
  • 摘要:We report a method of high-speed phase contrast and bright field microscopy which permits large cell culture vessels to be scanned at much higher speed (up to 30 times faster) than when conventional methods are used without compromising image quality. The object under investigation moves continuously and is captured using a flash illumination which creates an exposure time short enough to prevent motion blur. During the scan the object always stays in focus due to a novel hardware-autofocus system.
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