摘要：AgDNV is a powerful gene transduction tool and potential biological control agent for Anopheles mosquitoes. Using a GFP reporter virus system, we investigated AgDNV host range specificity in four arthropod cell lines (derived from An. gambiae , Aedes albopictus and Drosophila melanogaster ) and six mosquito species from 3 genera ( An. gambiae , An. arabiensis , An. stephensi , Ae. albopictus , Ae. aegypti and Culex tarsalis ). In vitro , efficient viral invasion, replication and GFP expression was only observed in MOS55 An. gambiae cells. In vivo , high levels of GFP were observed in An. gambiae mosquitoes. Intermediate levels of GFP were observed in the closely related species An. arabiensis . Low levels of GFP were observed in An. stephensi , Ae. albopictus , Ae. aegypti and Cx. tarsalis. These results suggest that AgDNV is a specific gene transduction tool for members of the An. gambiae species complex, and could be potentially developed into a biocontrol agent with minimal off-target effects.
