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  • 标题:Label-free single-cell protein quantification using a drop-based mix-and-read system
  • 本地全文:下载
  • 作者:Alireza Abbaspourrad ; Huidan Zhang ; Ye Tao
  • 期刊名称:Scientific Reports
  • 电子版ISSN:2045-2322
  • 出版年度:2015
  • 卷号:5
  • 期号:1
  • DOI:10.1038/srep12756
  • 语种:English
  • 出版社:Springer Nature
  • 摘要:Quantitative protein analysis of single cells is rarely achieved due to technical difficulties of detecting minute amounts of proteins present in one cell. We develop a mix-and-read assay for drop-based label-free protein analysis of single cells. This high-throughput method quantifies absolute, rather than relative, amounts of proteins and does not involve antibody labeling or mass spectrometry.
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