摘要：Water uptake is the fundamental requirement for the initiation and completion of seed germination that is a vital phase in the life cycle of seed plants. We found that seeds produced under four nitrogen levels showed significantly different germination speed. The objective of this study was to study the mechanism of rapid seed germination and explore which pathways and genes play critical roles in radicle protrusion. Anatomical data revealed that seed protein content affected endosperm structure of seeds. Moreover, scanning electron microscope maps showed that faster germinated seeds had a looser endosperm structure compared with other seeds. Subsequently, high throughout RNA-seq data were used to compare the transcriptomes of imbibed seeds with different germination speed. Gene ontology (GO) term enrichment analysis revealed that cell wall metabolism related genes significantly up-regulated in faster germinated seeds. In these genes, the top four were chitinase that had about fourfold higher expression in faster germinated seeds. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that faster germinated seeds had enhanced expression in glutathione metabolism. By combining these results, we propose a model for nitrogen fertilizer affects germination speed of wheat seed, which provide new insights into seed germination.
