摘要：There are large amounts of dissolved organic matter (DOM) released into the soil during spring thaw, but its bioavailability and components are still unknown. The quantity, composition and stability of DOM in water extracts of forest soils during thaw were studied after two-month freezing with 9 levels of soil moisture ranging from 10% to 90% water-filled pore space (WFPS), by measuring soil carbon dioxide (CO2) flux, biodegradable dissolved organic carbon (BDOC) and nitrogen (BDON), ultraviolet absorbance and parallel factor analysis of fluorescence excitation-emission matrices. Concentrations of BDOC, BDON, DOC and DON were lowest around 30% WFPS and relatively higher and lower soil moisture both increased DOM and BDOM concentrations in thawing soil. With increasing WFPS, the dominant component of soil DOM changed from humic acid-like substances to fulvic acid-like substances and the biological origin of DOM increased gradually. The protein-like component accounted for 8-20% of soil DOM and was affected by vegetation type and WFPS singly and interactively. The results implied that forest soils with more than 50% WFPS before winter freezing could release large amounts of fulvic acid-like DOM, which would be easily biodegraded and emitted as CO2 or run off with ground water during spring snow thaw.
