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  • 标题:Rapid de novo assembly of the European eel genome from nanopore sequencing reads
  • 本地全文:下载
  • 作者:Hans J. Jansen ; Michael Liem ; Susanne A. Jong-Raadsen
  • 期刊名称:Scientific Reports
  • 电子版ISSN:2045-2322
  • 出版年度:2017
  • 卷号:7
  • 期号:1
  • DOI:10.1038/s41598-017-07650-6
  • 语种:English
  • 出版社:Springer Nature
  • 摘要:We have sequenced the genome of the endangered European eel using the MinION by Oxford Nanopore, and assembled these data using a novel algorithm specifically designed for large eukaryotic genomes. For this 860 Mbp genome, the entire computational process takes two days on a single CPU. The resulting genome assembly significantly improves on a previous draft based on short reads only, both in terms of contiguity (N50 1.2 Mbp) and structural quality. This combination of affordable nanopore sequencing and light weight assembly promises to make high-quality genomic resources accessible for many non-model plants and animals.
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