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  • 标题:Fluorescence-based cell-specific detection for laser-capture microdissection in human brain
  • 本地全文:下载
  • 作者:Brad R. Rocco ; Hyunjung Oh ; Rammohan Shukla
  • 期刊名称:Scientific Reports
  • 电子版ISSN:2045-2322
  • 出版年度:2017
  • 卷号:7
  • 期号:1
  • DOI:10.1038/s41598-017-14484-9
  • 语种:English
  • 出版社:Springer Nature
  • 摘要:Cell-specific molecular investigations of the human brain are essential for understanding the neurobiology of diseases, but are hindered by postmortem conditions and technical challenges. To address these issues we developed a multi-label fluorescence in situ hybridization protocol and a novel optical filter device to identify cell types and control for tissue autofluorescence. We show that these methods can be used with laser-capture microdissection for human brain tissue cell-specific molecular analysis.
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