首页    期刊浏览 2024年11月06日 星期三
登录注册

文章基本信息

  • 标题:Fluorescent reporters for markerless genomic integration in Staphylococcus aureus
  • 本地全文:下载
  • 作者:Nienke W. M. de Jong ; Thijs van der Horst ; Jos A. G. van Strijp
  • 期刊名称:Scientific Reports
  • 电子版ISSN:2045-2322
  • 出版年度:2017
  • 卷号:7
  • 期号:1
  • DOI:10.1038/srep43889
  • 语种:English
  • 出版社:Springer Nature
  • 摘要:We present integration vectors for Staphylococcus aureus encoding the fluorescent reporters mAmetrine, CFP, sGFP, YFP, mCherry and mKate. The expression is driven either from the sarA-P1 promoter or from any other promoter of choice. The reporter can be inserted markerless in the chromosome of a wide range of S. aureus strains. The integration site chosen does not disrupt any open reading frame, provides good expression, and has no detectable effect on the strains physiology. As an intermediate construct, we present a set of replicating plasmids containing the same fluorescent reporters. Also in these reporter plasmids the sarA-P1 promoter can be replaced by any other promoter of interest for expression studies. Cassettes from the replication plasmids can be readily swapped with the integration vector. With these constructs it becomes possible to monitor reporters of separate fluorescent wavelengths simultaneously.
国家哲学社会科学文献中心版权所有