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  • 标题:Positron emission tomography reporter gene strategy for use in the central nervous system
  • 本地全文:下载
  • 作者:Tom Haywood ; Tom Haywood ; Corinne Beinat
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:2019
  • 卷号:116
  • 期号:23
  • 页码:11402-11407
  • DOI:10.1073/pnas.1901645116
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:There is a growing need for monitoring or imaging gene therapy in the central nervous system (CNS). This can be achieved with a positron emission tomography (PET) reporter gene strategy. Here we report the development of a PET reporter gene system using the PKM2 gene with its associated radiotracer [18F]DASA-23. The PKM2 reporter gene was delivered to the brains of mice by adeno-associated virus (AAV9) via stereotactic injection. Serial PET imaging was carried out over 8 wk to assess PKM2 expression. After 8 wk, the brains were excised for further mRNA and protein analysis. PET imaging at 8 wk post-AAV delivery showed an increase in [18F]DASA-23 brain uptake in the transduced site of mice injected with the AAV mice over all controls. We believe PKM2 shows great promise as a PET reporter gene and to date is the only example that can be used in all areas of the CNS without breaking the blood–brain barrier, to monitor gene and cell therapy.
  • 关键词:reporter gene ; positron emission tomography (PET) ; PKM2 ; [18F]DASA-23 ; adeno-associated virus
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