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  • 标题:Lipid and β-Carotene Production by Rhodosporidium diobovatum Cultured with Different Carbon to Nitrogen Ratios
  • 本地全文:下载
  • 作者:M. Mirzaie ; M. Mirzaie ; S.S. Saei-Dehkordi
  • 期刊名称:Journal of Food Quality and Hazards Control
  • 印刷版ISSN:2345-685X
  • 电子版ISSN:2345-6825
  • 出版年度:2019
  • 卷号:6
  • 期号:2
  • 页码:58-65
  • DOI:10.18502/jfqhc.6.2.956
  • 出版社:Shahid Sadoughi University of Medical Sciences, yazd, Iran
  • 摘要:Background: In food industry, carotenoids are used as food colorants conferring yellow to red color. This research was designed to study on lipid and β-carotene production by Rhodosporidium diobovatum cultured with different Carbon to Nitrogen (C/N) ratios. Methods: R. diobovatum was cultured in a medium containing 40 g/l glucose (as the carbon source) and different C/N ratios (20, 50, and 80), which were established by adding different amounts of (NH4)2SO4 (3.78, 1.51, and 0.94 g/l) as the source of nitrogen. High performance liquid chromatography, gas chromatography, and microplate reader were used to determine the glucose concentration, lipid production, and β-carotene concentration, respectively. Data were analyzed using IBM SPSS statistics (v. 24). Results: Cultures with a C/N ratio of 50 produced the greatest amount of lipids during 120 h pi. However, lipid synthesis in the first 48 h pi was very low for all three C/N ratios. Analyses of the lipid composition revealed that oleic acid and linoleic acid were the dominant (60%) fatty acids. Cultures with a C/N ratio of 50 also produced the greatest amount of β-carotene. Conclusion: R. diobovatum in the C/N of 50 culture medium resulted in greater concentrations of lipid and β-carotene. Defining the optimum C/N ratio will enable development of optimized bioprocess engineering parameters for scale-up production of lipid and β-carotene in food industries by this yeast species.
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