期刊名称：IOP Conference Series: Earth and Environmental Science
印刷版ISSN：1755-1307
电子版ISSN：1755-1315
出版年度：2019
卷号：252
期号：2
页码：1-7
DOI：10.1088/1755-1315/252/2/022019
出版社：IOP Publishing
摘要：In this study, the cultivated potato variety Xuanshu 2 was used as plant material, and MS was used as the basic medium, the in vitro regeneration culture of stem segments was carried out to study the optimal combination of phytohormone concentrations for callus induction and bud differentiation. The results indicated that the optimal medium for callus induction in stem segments was MS + 2.0 mg L−1 6-BA + 0.2 mg L−1 NAA, with 100% induction rate. The optimal medium for bud differentiation was MS + 2.0 mg L−1 6-BA + 2.0 mg L−1 ZT + 0.5 mg L−1 GA3, with 88% differentiation rate. In this study, the regeneration system of potato variety Xuanshu 2 was established, laying the foundation for the subsequent genetic transformation research of this variety.
