期刊名称：IOP Conference Series: Earth and Environmental Science
印刷版ISSN：1755-1307
电子版ISSN：1755-1315
出版年度：2019
卷号：252
期号：3
页码：1-8
DOI：10.1088/1755-1315/252/3/032111
出版社：IOP Publishing
摘要：In order to solve the problem of energy shortage and environmental pollution, eucalyptus is used as raw material to optimize the best process through dilute acid pre-treatment, enzymatic hydrolysis and fermentation, with xylose recovery rate, cellulose enzymatic hydrolysis rate, and total sugar yield as evaluation indicators. It has guiding significance for renewable biomass energy tree species to replace non-renewable petroleum-based raw materials to produce biobutanol, and the future development direction of biomass energy tree species production is looked forward. The pre-treatment process of eucalyptus with dilute sulfuric acid is optimized by response surface methodology. The effects of sulfuric acid concentration, solid-liquid ratio, reaction time and temperature on the pre-treatment effect are investigated with xylose recovery, cellulose enzymatic hydrolysis and total sugar yield as indicators. The results show that the pre-treatment effect of eucalyptus is the best when the concentration of H2SO4 is 0.52%(w/w), the pre-treatment temperature is 168 °C, and the time is 27min, and at this time, the xylose recovery rate is 87.58%. Then, under the optimum pre-treatment conditions, the effects of enzyme dosage and time on enzymatic hydrolysis are studied. The optimum enzymatic hydrolysis conditions are as follows: cellulase dosage 60FPU/g dry matter, and enzymatic hydrolysis for 36h. The cellulase hydrolysis rate can reach 90.82%, and the total sugar yield is 95.16%.
