期刊名称：IOP Conference Series: Earth and Environmental Science
印刷版ISSN：1755-1307
电子版ISSN：1755-1315
出版年度：2019
卷号：252
期号：4
页码：1-9
DOI：10.1088/1755-1315/252/4/042005
出版社：IOP Publishing
摘要：Porcine reproductive and respiratory syndrome virus (PRRSV) is a single-stranded and positive-sense RNA virus which can cause severe clinical disease and economic problem for worldwide pig industry. Droplet digital PCR (ddPCR) is an absolutely quantification method that can be used in viral detections. Here a one-step reverse transcription ddPCR was established for detection of highly pathogenic PRRSV (HP-PRRSV) and classical PRRSV. The assay was able to quantitatively detect HuN4 from 103 to 10−1 TCID50/mL. Through comparing with real-time quantitative reverse transcriptase PCR, the one-step RT-ddPCR was demonstrated more sensitive and can be used for the accurate quantification of PRRSV.
