期刊名称：IOP Conference Series: Earth and Environmental Science
印刷版ISSN：1755-1307
电子版ISSN：1755-1315
出版年度：2019
卷号：252
期号：4
页码：1-7
DOI：10.1088/1755-1315/252/4/042116
出版社：IOP Publishing
摘要：Bacteriophage ΦX174 specifically infects E.coli cells. It shares the similar biological characteristics with pathogenic virus. Thus, it is widely used as the "model virus" in the evaluation of virus control effect due to its safe operation. In this study, we have established a new bacteriophage detection method based on imagining flow cytometry. Combining the nucleic acid dyeing and capsid protein fluorescence labeling, the method based on imagining flow cytometry shows good linearity and specificity within its detection range. The establishment of this method is of great importance in fast and accurate detection for bacteriophage.
