期刊名称：IOP Conference Series: Earth and Environmental Science
印刷版ISSN：1755-1307
电子版ISSN：1755-1315
出版年度：2019
卷号：252
期号：5
页码：1-7
DOI：10.1088/1755-1315/252/5/052131
出版社：IOP Publishing
摘要：Ureolytic bacteria in 12 isolates, namely TCU1-TCU12, obtained from the soil with long-term application of urea fertilizer were screened and characterized. Urea degrading activity of all isolates was ranging from 2.4 to 22.9 mM. The ureolysis profiles of the isolates corresponded to fluctuations in pH value. Urease inhibitors, including acetohydroxamic acid and phenyl phosphorodiamidate, were applied to prove urea degrading activity. Moreover, the antifungal activity was verified by the TCU4 and TCU5 strains. The 16S rRNA gene sequence analysis revealed that among the 12 isolates, eight were Bacillus aryabhattai, three were Bacillus megaterium, and one was Bacillus subtilis. The random amplified polymorphic DNA was used to detect the genetic relationship among the 12 isolates. In addition, next-generation sequencing indicated that Proteobacteria was the most abundant phylum at 47.1% in the soil with long-term application of urea fertilizer. Bacillus species occupied 0.12%. Consequently, these ureolytic bacteria had the potential to be applied in the fields of concrete structure and medicine.
