标题：Utilization of Biourine and Arbuscular Mycorrhizal Fungi as Biotechnological Agents for Improving Land Productivity of Ex-Coal Mines and Testing Their Effects on the Content of Forage Fiber Fractions [ Hymenachne amplexicaulis (Rudge) Nees]
摘要：Background and Objective: Liquid biourine organic fertilizer can affect the physical, chemical and biological fertility of soil. Mycorrhizal biofertilizers can help plants to supply and absorb nutrients, especially phosphorus, which is low in availability in acidic/critical soils. The aim of this study was to characterize, test and prove that the administration of biourine liquid organic fertilizer and Arbuscular mycorrhizal Fungi biofertilizer (AMF) as a biotechnology agent, was able to improve the productivity of ex-coal mine lands and also affect the content of forage fiber feed fractions. Materials and Methods: An experiment using a completely randomized design with 4 types of treatment and 5 repetitions was conducted. The treatments consisted of: (1) Biourine concentration of 0%+AMF 20 g pot1, (2) Biourine concentration of 15%+AMF 20 g pot1, (3) Biourine concentration of 30%+AMF 20 g pot1 and (4) Biourine concentration of 45%+AMF 20 g pot1. Variables observed were Acid Detergent Fiber (ADF), Neutral Detergent Fiber (NDF), hemicellulose, cellulose and lignin. Results: The results of various analyses showed that the treatment had a highly significant effect (p<0.01) on all variables observed. The treatment with 45% biourine+AMF 20 g pot1 resulted in lower ADF, NDF and lignin contents and produced higher cellulose and hemicellulose content than the treatments with 30% biourine+AMF 20 g pot1, 15% biourine+AMF 20 g pot1 and 0% biourine+AMF 20 g pot1. Conclusion: Biourine liquid organic fertilizer and Arbuscular mycorrhizal Fungi biofertilizer (AMF) are biotechnology agents that improve the productivity of ex-coal mine land and also affect the content of forage fiber feed fractions.
