期刊名称：The Journal of the Korean Academy of Periodontology
印刷版ISSN：0250-3352
出版年度：2019
卷号：49
期号：4
页码：228-236
DOI：10.5051/jpis.2019.49.4.228
出版社：Korean Academy of Periodontology
摘要：Purpose The purpose of this study was to evaluate the synergistic effect of adjunctive hyperbaric oxygen (HBO) therapy on new bone formation and angiogenesis after 8 weeks of healing. Methods Sprague-Dawley rats (n=28) were split into 2 groups according to the application of adjunctive HBO therapy: a group that received HBO therapy (HBO group [n=14]) and another group that did not receive HBO therapy (NHBO group [n=14]). Each group was divided into 2 subgroups according to the type of bone graft material: a biphasic calcium phosphate (BCP) subgroup and an Escherichia coli -derived recombinant human bone morphogenetic protein-2-/epigallocatechin-3-gallate-coated BCP (mBCP) subgroup. Two identical circular defects with a 6-mm diameter were made in the right and left parietal bones of each rat. One defect was grafted with bone graft material (BCP or mBCP). The other defect was not grafted. The HBO group received 2 weeks of adjunctive HBO therapy (1 hour, 5 times a week). The rats were euthanized 8 weeks after surgery. The specimens were prepared for histologic analysis. Results New bone (%) was higher in the NHBO-mBCP group than in the NHBO-BCP and control groups ( P <0.05). Blood vessel count (%) and vascular endothelial growth factor staining (%) were higher in the HBO-mBCP group than in the NHBO-mBCP group ( P <0.05). Conclusions HBO therapy did not have a positive influence on bone formation irrespective of the type of bone graft material applied after 8 weeks of healing. HBO therapy had a positive effect on angiogenic activity.
关键词：Bone substitute; Biphasic calcium phosphate; epigallocatechin-3-gallate; Bone morphogenetic protein 2; Hyperbaric oxygen therapy
