摘要:This study aimed to produce microencapsulated probiotic Pseudoalteromonas piscicida (1Ub) and evaluate it with
prebiotic mannan-oligosaccharide (MOS) through the enrichment of Artemia sp., on bacterial population, growth
performances, immune responses, and disease resistance of Pacific white shrimp larvae. Microencapsulation
of probiotic was done by the freeze-drying method. The shrimp larvae were reared for 13 days and fed by the
Artemia sp. enriched with microcapsule of probiotic 1Ub (10 g/L), prebiotic MOS (12 mg/L), synbiotic, and
control without administration of microencapsulated probiotic and prebiotic, including negative (C-) and positive
(C+) control. On the day 14, all of the experimental shrimp larvae except C- were challenged through immersion
method with Vibrio harveyi MR5339 (107 CFU/mL). This study showed that the administration of microcapsule
of probiotic 1Ub, prebiotic MOS, and synbiotic through the enrichment of Artemia sp. could increase the bacteria
population, growth performances, immune responses, and disease resistance of Pacific white shrimp larvae.
Moreover, synbiotic treatment demonstrated the best result compared to other treatments.
其他摘要:ABSTRACT This study aimed to produce microencapsulated probiotic Pseudoalteromonas piscicida (1Ub) and evaluate it with prebiotic mannan-oligosaccharide (MOS) through the enrichment of Artemia sp., on bacterial population, growth performances, immune responses, and disease resistance of Pacific white shrimp larvae. Microencapsulation of probiotic was done by the freeze-drying method. The shrimp larvae were reared for 13 days and fed by the Artemia sp. enriched with microcapsule of probiotic 1Ub (10 g/L), prebiotic MOS (12 mg/L), synbiotic, and control without administration of microencapsulated probiotic and prebiotic, including negative (C-) and positive (C+) control. On the day 14, all of the experimental shrimp larvae except C- were challenged through immersion method with Vibrio harveyi MR5339 (10 7 CFU/mL). This study showed that the administration of microcapsule of probiotic 1Ub, prebiotic MOS, and synbiotic through the enrichment of Artemia sp. could increase the bacteria population, growth performances, immune responses, and disease resistance of Pacific white shrimp larvae. Moreover, synbiotic treatment demonstrated the best result compared to other treatments.
关键词:probiotic; prebiotic; synbiotic; Pacific white shrimp; microencapsulation
其他关键词:probiotic; prebiotic; synbiotic; Pacific white shrimp; microencapsulation