标题：Efficacy of Aeromonas hydrophila formalin-killed cells and lipopolysaccharides vaccines in maternal immunity of tilapia broodstock and the offspring resistance
摘要：The study aimed to analyze the efficacy of Aeromonas hydrophila formalin-killed cells (FKC) and lipopolysaccharides
(LPS) vaccines in maternal immunity of tilapia broodstock and the resistance of the offsprings. This study consisted
of two consecutive studies. Firstly, the broodstocks were vaccinated with PBS (non-vaccinated), FKC, LPS, and
combinations of FKC and LPS. Secondly, the offsprings from both the vaccinated and non-vaccinated broodstock
were vaccinated with the same vaccine for broodstock by immersion method. After vaccination, the offsprings were
challenged with pathogenic A. hydrophila. Antibody level and lysozyme were measured in the broodstock, egg, and
offsprings. In the first experiment, the result showed that vaccinated broodstock and their offsprings had delivered
a significant antibody level and lysozyme activity compared with the control. The highest relative percent survival
(RPS) of offspring resulted from the combination of FKC and LPS vaccinated broodstock at 5, 10, and 15 days
post-hatching at 85.00%, 75.23%, and 67.56%, respectively. The second experiment showed vaccinated offsprings
produced from vaccinated broodstock had a higher RPS than the RPS of vaccinated offsprings which produced
from non-vaccinated broodstock. In conclusion, vaccination that using a combination of FKC and LPS vaccine in
broodstock potentially improved the maternal immunity and protect their offspring from A. hydrophila infection.
其他摘要：ABSTRACT The study aimed to analyze the efficacy of Aeromonas hydrophila formalin-killed cells (FKC) and lipopolysaccharides (LPS) vaccines in maternal immunity of tilapia broodstock and the resistance of the offsprings. This study consisted of two consecutive studies. Firstly, the broodstocks were vaccinated with PBS (non-vaccinated), FKC, LPS, and combinations of FKC and LPS. Secondly, the offsprings from both the vaccinated and non‒vaccinated broodstock were vaccinated with the same vaccine for broodstock by immersion method. After vaccination, the offsprings were challenged with pathogenic A. hydrophila. Antibody level and lysozyme were measured in the broodstock, egg, and offsprings. In the first experiment, the result showed that vaccinated broodstock and their offsprings had delivered a significant antibody level and lysozyme activity compared with the control. The highest relative percent survival (RPS) of offspring resulted from the combination of FKC and LPS vaccinated broodstock at 5, 10, and 15 days post-hatching at 85.00%, 75.23%, and 67.56%, respectively. The second experiment showed vaccinated offsprings produced from vaccinated broodstock had a higher RPS than the RPS of vaccinated offsprings which produced from non-vaccinated broodstock. In conclusion, vaccination that using a combination of FKC and LPS vaccine in broodstock potentially improved the maternal immunity and protect their offspring from A. hydrophila infection.
