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  • 标题:Bioethanol Production from Banana Pseudostem by Using Separate and Cocultures of Cellulase Enzyme with Saccharomyces cerevisiae
  • 本地全文:下载
  • 作者:Hsin-Yi Huang ; Mahalli ; Joko Nurkamto
  • 期刊名称:Journal of Environmental Science and Technology
  • 印刷版ISSN:1994-7887
  • 电子版ISSN:2077-2181
  • 出版年度:2019
  • 卷号:12
  • 期号:4
  • 页码:157-163
  • DOI:10.3923/jest.2019.157.163
  • 出版社:Asian Network for Scientific Information
  • 摘要:Background and Objective: The lignocellulosic composition and availability of banana pseudostem is giving the selective choice for the renewable use of it from waste to valued product. The aim of the study was to improve bioethanol production by using separate and cocultures of cellulase enzyme with Saccharomyces cerevisiae . Materials and Methods: The raw material contains of 42.35% cellulose, 22.63% hemicellulose, 15.36% lignin, 8.79% extractives and 18.70% ash. Hot compressed water (HCW) and dilute H2SO4 pretreatment was performed to break down the lignin structure. Cellulase enzyme Aspergillus niger broken the β-glycosidic bonds and delivered the fermentable sugar, which was fermented to ethanol. The fermentation was carried out on the hydrolysate by cultured yeast ( S. cerevisiae from grapes). Results: The coculture fermentation in Simultaneous saccharification and fermentation (SSF) was found to be promising with maximum yield of ethanol (8.28 and 5.96 g L–1 of BP pretreated at 200°C and 0.5% of H2SO4, respectively) at 48 h than as compared to the separate hydrolysis and fermentation (SHF). Conclusion: The apparent result of the study observed that highest yield of ethanol was attained in a shorter duration in the coculture system containing cellulase enzyme and S. cerevisiae of grapes.
  • 关键词:Banana pseudostem; bioethanol; hot compressed water treatment; H2SO4 pretreatment; yeast culture; simultaneous saccharification and fermentation
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