期刊名称：International Journal of Conservation Science
印刷版ISSN：2067-533X
电子版ISSN：2067-8223
出版年度：2018
卷号：9
期号：2
页码：345-350
出版社：Alexandru Ioan Cuza University of Iasi
摘要：This paper represents our finding on new formulation of Ca-alginate beads preparation to support cell survival in the BPA biodegradation by extracellular laccase from Pseudomonas aeruginosa NR.22. The microencapsulation of the cells in Ca-alginate coated with 0.40% (w/v) chitosan has proved to enhance better survival in high BPA concentration (1200 ppm) with high activity and stable laccase production with BPA degradation over 80%. The coating has sized up the beads diameter to 116±0.01µm from 98µm (without the chitosan coating). The specific growth (?) of the Pseudomonas aeruginosa NR.22 in the Ca-alginate was 0.19 hour-1 suggesting that wellbeing of cells was excellent. The microcapsule yield (EY) was recorded as 94±0.01% proved that the bead's mechanical strength has been enhanced. Scanning Electron Microscope (SEM) resulted in very smooth non-pitting surface of Ca-alginate beads with chitosan coating. The coating process has increased the mechanical strength by 40% compares to non-coated beads which have ruptured slowly after 120 min. We are looking forward to research the mechanical properties of Ca-alginate over the residual stress and deformation of beads structure as an expansion of our academic report.
