期刊名称：International Journal of Conservation Science
印刷版ISSN：2067-533X
电子版ISSN：2067-8223
出版年度：2018
卷号：9
期号：3
页码：439-450
出版社：Alexandru Ioan Cuza University of Iasi
摘要：Accelerated Low-Water Corrosion (ALWC) leads to degradation of maritime steel structure and was grouped as one of microbial induced corrosion (MIC) type. This study was performed to isolate, identify, and characterized the growth of the microbes that has induced ALWC in order to decide for the best treatment using understanding of its potential EPS through biofilm formation. The isolation was done by using rust of the corrode metals collected from the steel structures and harbor walls which was exposed to the sea tides at the Port Kelang, Selangor, Malaysia. The rust collected was spread on Seawater Agar medium. Growth of five different species was recorded after 72 hours. The pure colonies were stained for Gram tests and were identified as Pseudomonas aeruginosa, Ochrobactrum anthropic, Pseudomonas luteola, Sphingomonas paucimobilis and Burkholderia cepacia using the APi KIT. The formation of the biofilm was studied to confirm the consortia-like-activity in the potential of causing the corrosion. Solid-state 13C NMR spectroscopy (SSNMR) of the EPS from consortia biofilm has revealed 3 types of EPS namely EPS1, EPS2 and EPS3 with all three contain different amount of microbiomolecules such as peptides, carbohydrates and lipids in different ratio. The data proposed a very strong environment and interrelation between the microbiomolecules of the biofilm formation; suggesting a strong biofilm activity was taking place at the maritime structure. Understanding the EPS and biofilm dynamics of the 5 species will assist in the design of engineered anti-corrosion formulation for the ALWC in the related marine structures. Addition of 10% of nitrifying bacteria has proven to rupture EPS1, EPS2 and EPS3 with more than 50% lost in the total concentration of peptide, carbohydrates and lipids. This result indicated that microbial based techniques can be further studied for the purposes of corrosion prevention.
