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  • 标题:Regenerative Competence in Root Explants of Rhynchostylis Gigantea, an Endangered Genera: An In Vitro Study
  • 本地全文:下载
  • 作者:V. Sharma
  • 期刊名称:International Journal of Conservation Science
  • 印刷版ISSN:2067-533X
  • 电子版ISSN:2067-8223
  • 出版年度:2019
  • 卷号:10
  • 期号:1
  • 页码:125-130
  • 出版社:Alexandru Ioan Cuza University of Iasi
  • 摘要:The neoformations in the in vivo root explants of Rhynchostylis gigantea depend upon their location, maturity level and chemical regime.The regeneration is affected by polarity showing basipetal gradient. The distal ones with intact tips with well developed root caps showed an extended growth with sub-apical formation of globular structure whereas,the proximal explants responded to the presence of cytokinin(Kn) in Mitra et al.,1976 medium.The effect of cytokinins was accentuated in the additional presence of NAA and the higher organogenetic responses are observed in explants when BAP,Kn was used in dose double than that of NAA.The regenerated plantlets were accilimatized &transfered to pots filled with moss, pine bark, brick and charcoal pieces (2:4:1:1) with 90% survival.
  • 关键词:Orchid; Flowering plants; Root; Tissue culture; Protocorm-like-bodies; Callus.
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