期刊名称：International Journal of Conservation Science
印刷版ISSN：2067-533X
电子版ISSN：2067-8223
出版年度：2019
卷号：10
期号：3
页码：485-492
出版社：Alexandru Ioan Cuza University of Iasi
摘要：Malaysian locally isolated Streptosporangium roseum FETL-88 (Sr-FETL88) survived thermo-induction from 50°C to 75°C and produced very stable biofilm formation at 75°C. Therefore, we are projecting our novel finding of the thermal-barrier-biofilm that help promoted for the cell survival. SEM has shown that Sr-FETL88 was flagellated; having biofilm formation other than producing positive black colonies on Congo Red Medium (CRM). We studied the thickness of the biofilm with temperature increment by using SEM. The thickest of biofilm formation of 2.04±0.03mm was recorded with temperature of 75°C. Biofilm components tested to reveal the highest content of EPS (55%, 5.0 X 1010cell/mL), lipid (25%, 17.8% of g/L dry weight) and various proteins (20%, 35.0g/mL). We have confirmed the findings by using ATR-FTIR spectra result. CLSM analysis of 24 hour biofilms of Sr-FETL88 grown at 75°C has been done with single section (z = 1μm) through a micro-colony growth of rod-shaped cells with the EPS stained with LPA lectin in green fluorescence. It is confirmed that the biofilm has been used by our Sr-FETL88 as a thermal-barrier to survive 75°C, a rare growth temperature for a Malaysian locally isolated species.
关键词：Biofilm; Streptosporangium roseum; Thermo-barrier; Protein thermostability
